Clonal expansion of cells for generation of cell lines
For clonal expansion of cells, the Puncher system as well as the Pump-unit are placed inside a laminar flowhood to ensure sterility. The flow hood is designed according the requirements of the Puncher system. A computer screen is mounted in the back of the flow hood to facilitate cleaning and keep an undisturbed flow. Keyboard and mouse have a silicon flat surface to enable easy cleaning with all hospital grade disinfectants.
Puncher system is fully automated
The Puncher system contains high end scanning stages to quickly position the chip, wells plate and punch needle. Slides with the microwell chip, Punch needles and tissue culture plates are easily docked on the system. After imaging the cells of interest are presented in a gallery that is automatically generated. The selected cells are isolated by punching the bottom and cell from the microwell towards a culture plate for clonal cell expansion.
The system uses a Lumencor LED excitation light source and Hamamatsu CMOS scientific camera. This enables the user to acquire high quality fluorescence images of the single viable cells with a 10x, 20x or 40x objective.
| Base: | Inverted Nikon Ti2 microscope |
| Objectives: | 10x NA 0.30, 20x NA 0.45, 40X NA 0.65 |
| Filters cubes: | DAPI, FITC, PE, APC, Reflection |
| LED: | Sola Lumencor Light source |
| Camera: | Hamamatsu CCD camera, Orca Flash 4.0 Hamamatsu CCD camera, Orca Flash 4.0 LT, type C11440-42 |
| Data management: | VyCAP imaging and Puncher system software |
| Application: | Enumeration, single cell isolation and clonal expansion |
| Imaging time per slide: | Typically 10-20 min |
| Transfer: | 1 cell/second |
| Collection tubes: | 96 well TCP plates |
| Flowhood: | Telstar class IIB (height=2285mm, width=1630mm,depth=1000mm) |
