Single cell isolation for clonal expansion
Select individual viable cells for single cell isolation, followed by clonal expansion, using the single cell disposable. The single cell disposable contains a sterile slide with a microwell chip. To distribute the single cells the disposable is connected to the Pump-unit. Image based cell selection and isolation is performed using the VyCAP Puncher system.
Distribute one cell in one microwell
The single cell disposable contains a slide with a microwell chip to distribute single cells. This chip contains 6.400 microwells. Each microwell has a diameter of 70 µm and the bottom contains a single pore with a diameter of 5µm. The Pump unit creates a small negative pressure at the waste side of the disposable. Fluid enters the microwell and exists through the single pore at the bottom of each microwell. After a cell has landed onto the pore the flow stops and no other cell will enter the same well. The next cell is diverted to a neighboring well. In this manner single cells are sorted in individual wells across the entire microwell chip in a few minutes. During sorting the cells typically resides in the culture medium.
Select and single cell isolation with Puncher system
Single cells isolation is automated using the VyCAP Puncher system. Using this system the single cells are punched from the microwelll chip to the culture plate for further expansion. During this process the cells remain in culture medium at all times.
|Slide (lxb):||57x24mm or 75x24mm|
|Slide material:||HIPS / black|
|Chip size (lxb):||10x10mm|
|Pore diameter:||5µm (other pore sizes are available on request)|
|Number of microwells:||6400|
|Filter membrane material:||Silicon nitride, thickness 1 µm|