Fluorescence labeling of collected cells
Label filtered CTC and other cells easily with the easy to use VyCAP Cellstainer. After the sample volume is processed, the slide with the microsieve is transferred manually to the Cellstainer. The Cellstainer uses absorbers to remove excess reagents and facilitate washing, removing the need of any centrifugation steps that could yield cell loss. A maximum volume of 1ml can be used per absorber.
Label CTCs with fluorescent markers
To label circulating tumor cells a combination of fluorescence labels is applied to the collected cells on the filter. After incubation, the microsieve is pushed onto the absorber to remove excess reagents. Labelling protocols for detection are available on our website. The protocol for the enumeration of CTC uses the following set of immuno-fluorescence labels:
- Anti Cytokeratines – Phycoerythrin (PE)
- Anti CD45 – Allophycocyanin (APC)
- Anti CD16 – Allophycocyanin (APC)
Use your own fluorescent labels
VyCAP does not provide dedicated staining kits. Users can use their own labels and reagents of choice as the protocols are easy to adjust to your own needs. Besides immunofluorescence labels, FISH probes can be applied as well.
Cellstainer absorbers are suplied with each ordered filtration disposable. The absorber is manually placed in the Cellstainer.